cre小鼠与flox杂交
![如何成为一个合格的小鼠铲屎官](https://imgs.wantubizhi.com/img/ADDA3C5D760C6828B77B505A29D43E8325910CB6A9A05E2ADF00C4C0C5FE5C2DD7CBD44E4A8CFD80EED105E03F71864C24D0B9914CB1FD905B9850515B2729D050069BCD9883F133308DEB3B2FCF3864A4E388E5FDCE9707CFC132FCF825C358)
如何成为一个合格的小鼠铲屎官
图片尺寸2008x2052![将vegfr2fl/fl小鼠与villin-cre小鼠杂交,产生肠上皮细胞中缺乏vegfr2](https://imgs.wantubizhi.com/img/A1A61BAA66193547F3A6DD7D8EE527E9997277FDBC29F41D1C5DEF6A67B80A4740DA7B5FFBA9484538C95F5536F03D9BA4BB78068DFC86EBB5A65F82C78DE61220860FBD7B8C533A7E66CAC7457281873156D317588B40AE5647AE532732FDD6)
将vegfr2fl/fl小鼠与villin-cre小鼠杂交,产生肠上皮细胞中缺乏vegfr2
图片尺寸1037x866![(3)条件性基因敲除获得 flox 纯合同时 cre 阳性的小鼠:(f/ ;cre-)](https://imgs.wantubizhi.com/img/A1A61BAA66193547F3A6DD7D8EE527E9E6F02EB536C738226EB2CA20B7278DCA9639FDADC595EA30875B23D6D0476706C2A1E2948478000D318D12B97664FAB4236FE17152344AE47279379C4367330C30A9675026202E56A4CE75AF04FA34E6)
(3)条件性基因敲除获得 flox 纯合同时 cre 阳性的小鼠:(f/ ;cre-)
图片尺寸1080x801![条件性敲除小鼠的原理,构建与应用](https://imgs.wantubizhi.com/img/735A5D1F980B52A636CC46F12F2D1A73622BB61D994AAA75F92A588092B20DFE4103CF0277FF2401E5F0F8C2B6BA863A9733FA099B53F7AE415FF17CB394BA7C7406834831E4C58C332FC432CDA9DF8C7EB4061A89DF41FDB04CC195607FD842)
条件性敲除小鼠的原理,构建与应用
图片尺寸521x616![crisprcas9基因敲除敲入鼠构建](https://imgs.wantubizhi.com/img/ADDA3C5D760C6828B77B505A29D43E83B6B17463E430BDECADBDF40A26166D446DA2EB5448F84C529EBD70C49B89648976DC2E4BB91B5A3E1A13989F5907BEB8434433857733AC0C5DA0D1B9103C54E9AEBF720486F58979B0A48C6DEA0FC36F)
crisprcas9基因敲除敲入鼠构建
图片尺寸558x528![与野生型小鼠杂交:阳性crispr-pro小鼠( /flox)与野生型小鼠( / )杂交](https://imgs.wantubizhi.com/img/A1A61BAA66193547F3A6DD7D8EE527E9B1E394C35346F3ACFFD9E1EB18D4F387036E5D5F35B966C3B7762F2533D5A9F96EBAD2C87682B1DD62F0D25B58A47BEA95D9DE15333FF05E7B917B47FFF1ABA6F8958F0A4671E1DBCBD04B3473CD9E5D)
与野生型小鼠杂交:阳性crispr-pro小鼠( /flox)与野生型小鼠( / )杂交
图片尺寸1280x970![crispr-pro小鼠与野生型小鼠( / )杂交,获得f1代杂合子小鼠( /flox)](https://imgs.wantubizhi.com/img/ADDA3C5D760C6828B77B505A29D43E837521FC6B3210351ED4C9FC458FE77DA25A7BBD0C8A9BB0F0DEC77BC91B61E5D82506AD565BD4B3BE1363D9FFE7C0044F97E1978624A1697F4ABD9AC1CE0CB9DD9721A41A54EF80E9B5776FA2516E7F5B)
crispr-pro小鼠与野生型小鼠( / )杂交,获得f1代杂合子小鼠( /flox)
图片尺寸600x466![纯合flox的鼠(因为我要把两个染色体都敲除),同时还要带有一个cre基因](https://imgs.wantubizhi.com/img/A1A61BAA66193547F3A6DD7D8EE527E962928FC3B05F0E3A9632AF86AA0D35E028FD66652965DEEB2E180CE9BAADA12940784972BE0533D9A94A62F587EFE0A04C672C0968DA8E0DCE09741292C71AA2DFD8185AB5A8CD300B8155392D33CDC6)
纯合flox的鼠(因为我要把两个染色体都敲除),同时还要带有一个cre基因
图片尺寸554x238![小鼠大学问诱导型cre给基因敲除装个开关](https://imgs.wantubizhi.com/img/ADDA3C5D760C6828B77B505A29D43E835FADB6D44DD1D4E7A1788613A2791D5B582ABD3CE916DBE39C74B9A790719FB4D53EE4B802F83CE5589B158B9A73570C25243BBFE436ED195159A50F98F016A630E7F42413C1F03DBC658F7AA076E57D)
小鼠大学问诱导型cre给基因敲除装个开关
图片尺寸362x496![纯合flox的鼠(因为我要把两个染色体都敲除),同时还要带有一个cre基因](https://imgs.wantubizhi.com/img/735A5D1F980B52A636CC46F12F2D1A73C778174A9321B7C36CEC030F91CA590F5DAF597FF371755C6FDA5D722B5DA3C745F2650A7203829FFC81BAF48697BCD075707EA0E707E2617F4EC58DD07B0604CA8A9C794DD8257082B608E7210C0969)
纯合flox的鼠(因为我要把两个染色体都敲除),同时还要带有一个cre基因
图片尺寸521x443![(3)条件性基因敲除获得 flox 纯合同时 cre 阳性的小鼠:(f](https://imgs.wantubizhi.com/img/A1A61BAA66193547F3A6DD7D8EE527E9A2068667FE70644112CB8632172ED108BFE5DC7EAB9C8A211F4588F23A853AA6911C7C81DF942C8777E9743D1D28FF7D1BC437C279119D8415CF604C825F7B40DFA93DA9679307E030B456F7EFA770AD)
(3)条件性基因敲除获得 flox 纯合同时 cre 阳性的小鼠:(f
图片尺寸1080x699![cre 阳性的小鼠(f/f;cre-)× (f/ ;cre ) → f/f;cre flox 小鼠在去除](https://imgs.wantubizhi.com/img/A1A61BAA66193547F3A6DD7D8EE527E96EF4F1BF0E40AF001B89E44232697FFD0D45ACEB59691B539A3563AF8375AFC3DB945FCF3B86CA94DFD33B17CF07B83D603C7F68E67FA5874FC404791E06BA190B4C7448A9A74C1CA191066D92D795E1)
cre 阳性的小鼠(f/f;cre-)× (f/ ;cre ) → f/f;cre flox 小鼠在去除
图片尺寸823x550![> 制备条件型基因敲除小鼠](https://imgs.wantubizhi.com/img/ADDA3C5D760C6828B77B505A29D43E838C2E10DD73B93E282AC402035EEA64B62BD0E5F4E345669FC45E2D9946662DAEAA95C0846378F58F797408E22BD7AE8FDA0BF4F297B38AD7DE97195D48785EDD9A5D670DE1B4E55349B41FA1EE60CF39)
> 制备条件型基因敲除小鼠
图片尺寸670x300![cre 阳性的小鼠(f/f;cre-)× (f/ ;cre ) → f/f;cre flox 小鼠在去除](https://imgs.wantubizhi.com/img/A1A61BAA66193547F3A6DD7D8EE527E91C6651E706E087F31108333295F65FDBD962B956FD8E6D43B1E252763B7B73EE301855AD37D9863C10CBF7E3C61BC70E76AC7D49BC5828ABD4CD4ED9A76E92D43FBE6DAB2EF4D59CA4A7650FE172F584)
cre 阳性的小鼠(f/f;cre-)× (f/ ;cre ) → f/f;cre flox 小鼠在去除
图片尺寸841x810![师妹:师兄,我应该用雄性 cre 配雌性 flox 小鼠还是用雌性 cre 配雄性](https://imgs.wantubizhi.com/img/ADDA3C5D760C6828B77B505A29D43E836830AE0DE93A78175196BA0800DC92F09F2295D1D7E85F8B897345653C645CD209E1C96F223E7DEEFEFD852F277044E2B3952C4EB1603FB60D8FBCBFDD30FE5F2565D509F814389F48D11DB5E9005DED)
师妹:师兄,我应该用雄性 cre 配雌性 flox 小鼠还是用雌性 cre 配雄性
图片尺寸788x526![将f3代小鼠进行繁殖,2种方案:flox/flox,cre x flox/flox,cre-;flox](https://imgs.wantubizhi.com/img/ADDA3C5D760C6828B77B505A29D43E836E3A28ECAB45F232F1F1239FDAA1753657F5C52FA16D1BB7F4344F842E10C8522C9FF49A52044DC4A7CDB12CFD9247C3AF14AE2DBEC893DA6C58A6DD537C1A4B4B9B81AF9EF84327D0DA4A9166645A26)
将f3代小鼠进行繁殖,2种方案:flox/flox,cre x flox/flox,cre-;flox
图片尺寸786x398![的两侧(称为"floxing",即"side by loxp")将允许基因表达,直到cre出现](https://imgs.wantubizhi.com/img/A1A61BAA66193547F3A6DD7D8EE527E9888CB3742125C7E24140BEE1755C4CD8D72A9B2321367F409D4EB340203B35470EF96E486337FF7623D5067523CB736A58B348BCC0F0899AC3577BCD7CFD0EAA76475CA9F123F4B928EB37ABB14279B7)
的两侧(称为"floxing",即"side by loxp")将允许基因表达,直到cre出现
图片尺寸650x517![crechn那么获得目的ko鼠概率也不一样,同时获得同窝对照小鼠wt概率](https://imgs.wantubizhi.com/img/735A5D1F980B52A636CC46F12F2D1A73C041B7FEBC32236D6D1A54285786679143D529D9546BC0AF1A8610A5591EC5418FD5E4E87C40F851B7D37722398C0E9FEF017D1E7BA667980B96F7316E824E981A3E238ECC4E76D1B0B452A98B08045B)
crechn那么获得目的ko鼠概率也不一样,同时获得同窝对照小鼠wt概率
图片尺寸1080x1397![真没想到用了它不到1个月就拿到了基因敲除小鼠](https://imgs.wantubizhi.com/img/735A5D1F980B52A636CC46F12F2D1A7364B409DC0135118FB9D5353D0C5A72170B818B80CE7DDAC780DFBA3570B65D807768580A441D91C4E9AE48F426B7A3F9A807CE3E7075D94202A94C8781573BFA6AD641E62232A1CF8DE247F6DD2DB1DE)
真没想到用了它不到1个月就拿到了基因敲除小鼠
图片尺寸1384x492![基因敲除,以cre为主](https://imgs.wantubizhi.com/img/735A5D1F980B52A636CC46F12F2D1A737B05A7583F6CF99EEF335376B68C369CF06ECFDBD9510D66D478EA90EFBEA3678AEFDA80CD28022DECFC4153F028CD136F33F0F8656AECB31C55AD782DFB31BB64D484B42CDE52B4E44A409782E3006F)
基因敲除,以cre为主
图片尺寸453x325